Plant Regeneration from Cotyledon Explants of Bittergourd as influenced by Adenine Sulphate and D-Biotin
نویسندگان
چکیده
The first experiment was done to select the most suitable explant establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP 0.2 NAA. Cotyledon responsive which exhibited quick callus initiation. second conducted determine effect of media BAP, NAA also various concentrations (0, 20, 40 60 l-1) adenine sulphate (AS). best formation with AS. Callus derived particular showed positive response regeneration after subculturing 2.0 In third experiment, D-biotin added into composition had beneficial callogenesis association Regenerated shoots multiplied 3.0 BAP. Eventually, rooting achieved included IBA.
منابع مشابه
Somatic Embryogenesis and Plant Regeneration from Embryonic Axes and Cotyledons Explants of Tea (Camellia sinenesis L.)
In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on Murashige and Skoog (MS) medium containing 0, 1 and 5 mM 2, 4-D alone for embryonic axes and 0, 1 and 5 mM 2, 4-D along with 0...
متن کاملShoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don
The gesneriaceous perennial plant, Lysionotus serratus, has been used in traditional Chinese medicine. It also has a great development potential as an ornamental plant with its attractive foliage and beautiful flowers. An efficient propagation and regeneration system via direct shoot organogenesis from leaf explant was established in this study. High active cytokinin (6-benzyladenine (BA) or th...
متن کاملCharacterization of CDKA gene expressed during in vitro regeneration from pepper (Capsicum annuum L.) cotyledon explants
Adventitious bud-like structures were directly regenerated from pepper (Capsicum annuum L. cv. Baklouti) cotyledon explants taken from 14 days-old seedlings and cultivated on MS medium supplemented with 5.7 μM IAA and 8.8 μM BAP. Histological and molecular studies were performed at different stages of the in vitro culture process in order to follow the regeneration pathway. Histological study r...
متن کاملHigh-Frequency in Vitro Direct Shoot Regeneration from Nodal Explants of Hyssop Plant (Hyssopus officinalis L.)
Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitro culture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of TDZ and BAP in combination with 1 µmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01...
متن کاملInduction of Callus and Somatic Embryogenesis from Cotyledon Explants of Fagonia indica Burm.
To explore the potential for in vitro rapid regeneration of Fagonia indica Burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on MS medium which supplemented with 1.0-5.0 µM 2,4-D. Callus was initiated from cotyledon explant on MS medium supplemented in all concentrations of 2,4-D investigated. 2,4-D was more effective than IAA and NAA for cal...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Food and Agriculture
سال: 2023
ISSN: ['1800-4288', '2613-8530']
DOI: https://doi.org/10.4038/jfa.v16i1.5280