Plant Regeneration from Cotyledon Explants of Bittergourd as influenced by Adenine Sulphate and D-Biotin

نویسندگان

چکیده

The first experiment was done to select the most suitable explant establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP 0.2 NAA. Cotyledon responsive which exhibited quick callus initiation. second conducted determine effect of media BAP, NAA also various concentrations (0, 20, 40 60 l-1) adenine sulphate (AS). best formation with AS. Callus derived particular showed positive response regeneration after subculturing 2.0 In third experiment, D-biotin added into composition had beneficial callogenesis association Regenerated shoots multiplied 3.0 BAP. Eventually, rooting achieved included IBA.

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ژورنال

عنوان ژورنال: Journal of Food and Agriculture

سال: 2023

ISSN: ['1800-4288', '2613-8530']

DOI: https://doi.org/10.4038/jfa.v16i1.5280